Composite

Part:BBa_K566042:Design

Designed by: Porfirio Quintero-Cadena, José Eduardo Almeyda Carbajal   Group: iGEM11_UANL_Mty-Mexico   (2011-09-28)


composite Biphasic switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2454
    Illegal BglII site found at 2510
    Illegal BglII site found at 2684
    Illegal XhoI site found at 2548
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 547
    Illegal AgeI site found at 558
    Illegal AgeI site found at 1959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1450


Design Notes

Search for illegal sites and it was optimized for E.coli codon usage


Source

Planning

References